SwitchGear also offers functionally validated sets of promoter and 3′UTR reporter constructs for measuring a variety of biological responses. Understand the basics of 3′UTR reporter experiments. Understand the basics of promoter reporter experiments. Our GoClone ® Collections include more than 18,000 human promoters and 12,000 3′UTRs cloned into LightSwitch reporter vectors (Figure 1). GoClone ® reporter vectors are transfection-ready vectors, so no cloning or DNA preparation is required. LightSwitch™ Promoter and 3′UTR GoClone Collections The system includes a novel luminescent reporter gene (RenSP) and vector, an optimized companion assay reagent, and pre-cloned promoters and 3′UTRs. The LightSwitch™ Luciferase Assay System is a unique collection of over 30,000 cloned regulatory elements from the human genome.
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